S3396
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 | Immunoblotting Erythrocytes ghost extract was separated on SDS-PAGE and probed with Monoclonal AB To Spectrin (α+β) (MO) CL:SB-SP1 (Prepack) (Cat. No. S3396). The antibody was developed with HRP APA Mouse Fab ads/HIgG (Cat. No. A9917) and AEC substrate. Antibody dilution 1/1,000. |
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S3396
Sigma
Monoclonal Anti-Spectrin (α and β) antibody produced in mouse
clone SB-SP1, ascites fluid
| MDL number: | MFCD00164739 |
Description
| Immunogen | human erythrocyte spectrin. |
| Specificity | This antibody reacts specifically with the α and β chains of human erythrocyte spectrin. |
Properties
| antibody form | ascites fluid |
| clone | SB-SP1 (monoclonal) |
| contains | 15 mM sodium azide |
| species reactivity | human |
| application(s) | immunoblotting: 1:500 using extract of human erythrocyte ghosts or purified human erythrocyte spectrin |
| indirect immunofluorescence: suitable using erythrocyte smears | |
| microarray: suitable | |
| isotype | IgG1 |
| Gene Information | human ... SPTA1(6708), SPTB(6710), SPTBN1(6711) |
| shipped in | dry ice |
| storage temp. | −20°C |
Safety
| WGK Germany | NWG |
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References
| reference | Dietrich, G., et al., Indicators of erythrocyte damage after microwave warming of packed red blood cells. Clin. Chem. 49, 792-799, (2003) Abstract |
| Chou, D.E., et al., α-Adducin dissociates from F-actin and spectrin during platelet activation J. Cell Biol. 161, 557-570, (2003) Abstract | |
| Dupuy, A.D., et al., Protein area occupancy at the center of the red blood cell membrane. Proc. Natl. Acad. Sci. U. S. A. 105, 2848-2852, (2008) Abstract |
